Publications
2026
Halder, A., Carey, S.C., Jeong, J.-H., Shapla, U.M., Clewett, C.F.M., Cavagnero, S. Hypersensitive Detection of Neurotransmitters in Biological Media by Optically Enhanced Benchtop NMR Spectroscopy. Submitted (2026).
Masse, M.M., Hutchinson, R.B., Morgan, C.E., Millan, N., Das, J., Allaman, H.J., Guan, H., Yu, E.W., Cavagnero, S. Mapping Protein-Protein Interactions at Birth: Single-Particle Cryo-EM Analysis of a Ribosome/Nascent-Globin Complex. ACS Centr. Sci. additions and corrections (2026).
Shreeve, K., Jang, J., Srivathsan, M., Li, S., Scott, T.D., Cavagnero, S. Prediction of Prion Proteins in E. Coli Based on Bimodal Sequence Characteristics. Proteins Struct. Funct. Bioinf. 1-20 (2026).
Jeong, J.-H., Halder, A., Cavagnero, S. Nuclear-Spin Hyperpolarization of Biomolecules by Optically Enhanced NMR at Ultra-High Field. J. Chin. Chem. Soc. 1–17 (2026).
Li, S., Yang, H., Hofstetter, H., Tonelli, M., Cavagnero, S. Magnetic-Field Dependence of LC-Photo-CIDNP in the Presence of Target Molecules Carrying a Quasi-Isolated Spin Pair. Appl. Magn. Res. 57:10, correction (2026).
2025
Shapla, U., Smith, J.L., Halder, A., Thompson, L., Buller, A. R., Cavagnero, S. Highly Sensitive Detection of Tyrosine and Neurotransmitters by Stereoselective Biosynthesis and Optically Enhanced NMR. J. Am. Chem. Soc. 147, 42361-42374 (2025).
O’Connor, M.S., Konovalov, K.A., Duvall, J.L., Jang, J., Lao, Y. Cavagnero, S., Huang, X. Role of Hsp70 Chaperone in Protein Folding Elucidated by Markov State Modeling and MD Simulations. Biophys. J. 125, 1-14 (2025).
Cavagnero, S. Insights into Life Under Extreme Conditions: How Does It Happen and Why Do We Care?. BPS Bulletin, January issue (2025).
2024
Yang, H., Li, S., Mickles, C.A., Guzman-Luna, V., Sugisaki, K., Thompson, C.M., Dang, H.H., Cavagnero, S. Selective Isotope Labeling and LC-Photo-CIDNP Enable NMR Spectroscopy at Low-Nanomolar Concentration. J. Am. Chem. Soc. 146, 32160-32160, Additions and Corrections (2024).
Li, S., Yang, H., Hofstetter, H., Tonelli, M., Cavagnero, S. Magnetic-Field Dependence of LC-Photo-CIDNP in the Presence of Target Molecules Carrying a Quasi-Isolated Spin Pair. Appl. Magn. Res 56, 427, correction (2024).
Masse, M.M., Guzman-Luna, V., Varela, A.E., Hutchinson, R.B., Shapla, U., Srivastava, A., Wei, W., Fuchs, A.M., Cavagnero, S. Nascent Chains Derived from a Foldable Protein Sequence Interact with Specific Ribosomal Surface Sites near the Exit Tunnel. Sci. Rep. 14, 12324 (2024).
Masse, M.M., Hutchinson, R.B., Morgan, C.E., Allaman, H.J., Guan, H., Yu, E.W., Cavagnero, S. Mapping Protein-Protein Interactions at Birth: Single-Particle Cryo-EM Analysis of a Ribosome/Nascent-Globin Complex. ACS Centr. Sci. 10, 385-401 (2024).
Cavagnero, S. Biological Helpers at the Molecular Level: Cell Stress, Environmental Factors and Chaperones. BPS Bulletin, March issue (2024).
Li, S., Bhattacharya, S., Chou, C.-Y., Chu, M., Chou, S.-C., Tonelli, M., Goger, M., Yang, H., Palmer, A.G., Cavagnero, S. LC-Photo-CIDNP Hyperpolarization of Biomolecules Carrying a Quasi-Isolated Spin Pair: Magnetic-Field Dependence via a Rapid-Shuttling Device. J. Magn. Res. 359, 107616 (2024).
2023
Cavagnero, S., Schanda, P. JMR/JMRO/JSB/JSBX Special Issue on the Nuclear Magnetic Resonance of Biological Systems. Editorial, J. Magn. Res., 356, 107562 (2023).
Addabbo, R.M., Hutchinson, R.B., Allaman, H.J., Dalphin, M.D., Mecha, M.F., Yue Liu, Y., Staikos, A., Cavagnero, S. Critical Beginnings: Selective Tuning of Solubility and Structural Accuracy of Newly Synthesized Proteins by the Hsp70 Chaperone System. J. Phys. Chem B, 127, 3990-4014 (2023).
Eills, J., Blanchard, J., Budker, D., Cavagnero, S., Chekmenev, E., Elliott, S., Jannin, S., Lesage, A., Matysik, J., Meersmann, T., Prisner, T., Reimer, J., Yang, H., Koptyug, I. Spin Hyperpolarization in Modern Magnetic Resonance. Chem. Rev. 123, 1417-1551 (2023).
Chen, X. Hutchinson, R.B., Cavagnero, S. Distribution and Solvent-Exposure of Hsp70 Chaperone Binding Sites across the E. Coli Proteome. Proteins: Struct. Funct. Bioinf. 91, 665-678 (2023). (*) equal contributors.
Cavagnero, S. Emerging Tools in Biophysics: Stem Cells, Organoids and Bioprinting. BPS Bulletin, January issue (2023).
2022
Li, S., Yang, H., Hofstetter, H., Tonelli, M., Cavagnero, S. Magnetic-Field Dependence of LC-Photo-CIDNP in the Presence of Target Molecules Carrying a Quasi-Isolated Spin Pair. Appl. Magn. Res. 54, 59-75 (2022).
Yang, H., Li, S., Mickles, C.A., Guzman-Luna, V., Sugisaki, K., Thompson, C.M., Dang, H.H., Cavagnero, S. Selective Isotope Labeling and LC-Photo-CIDNP Enable NMR Spectroscopy at Low-Nanomolar Concentration. J. Am. Chem. Soc. 144, 11608-11619 (2022).
Mecha, M.F., Hutchinson, R.B., Lee, J.H., Cavagnero, S. Protein Folding in Vitro and in the Cell: from a Solitary Journey to a Team Effort. Biophys. Chem. 287, 106821 (2022).
Bondos, S. Cavagnero, S. Regaining and Preserving Wellness during and after the COVID-19 Pandemic. BPS Bulletin, May issue (2022).
Cavagnero S., Guzman-Luna, V. Mg+2 Ions Mediate the Interaction of Intrinsically Disordered Nascent Chains with the Ribosome. Neurosci. Chron. 3, 3-5 (2022).
Cavagnero, S. Understanding and Curing Deadly Proteinopathies by Harnessing the Power of Biophysics. Biophys. Soc. Newslett., January issue (2022).
2021
Guzman-Luna V., Fuchs, A., Allen, A.J., Staikos, A., Cavagnero, S. An Intrinsically Disordered Nascent Protein Interacts with Specific Regions of the Ribosomal Surface near the Exit Tunnel. Commun. Biol. 4, 1236 (2021).
Hutchinson, R.B., Chen, X., Zhou, N., Cavagnero, S. Fluorescence Anisotropy Decays and Microscale-Volume Viscometry Reveal the Compaction of Ribosome-Bound Nascent Proteins. J. Phys. Chem. B 125, 6543-6558 (2021).
Yang, H., Mecha, M.F., Goebel, C.P., Cavagnero, S. Enhanced Nuclear-Spin Hyperpolarization of Amino Acids and Proteins via Reductive Radical Quenchers. J. Magn. Res. 324, 106912 (2021).
2020
Cavagnero, S. Celebrating Science Wizardry and Protein Design at SACNAS. BPS Bulletin, December issue (2020).
Addabbo, R.M., Dalphin, M.D., Mecha, M.F., Liu, Y., Staikos, A., Guzman-Luna, V., Cavagnero, S. Complementary Role of Co- and Post-Translational Events in De Novo Protein Biogenesis. J. Phys. Chem. B 124, 6488-6507 (2020).
Thompson, C. M.; McDonald, A. D., Yang, H., Cavagnero, S. Buller, A. R. Modular Control of L-Tryptophan Isotopic Labeling via an Efficient Biosynthetic Cascade. Org. Biomol. Chem. 18, 4189-4192 (2020).
Lee, J., Park, S.H., Cavagnero, S., Lee, J.H. High-Resolution Diffusion Measurements of Proteins by NMR under Near Physiological Conditions. Analyt. Chem. 92, 5073-5081 (2020).
Yaeger-Weiss, S.K., Jennaro, T.S., Mecha, M.M., Becker, J.H., Cavagnero, S. Net Charge and Nonpolar Content Guide the Identification of Folded and Prion Proteins. Biochemistry 59, 1881-1895 (2020).
Dalphin, M.D., Stangl, A.J., Liu, Y., Cavagnero, S. KLR-70: a Novel Cationic Inhibitor of the Bacterial Hsp70 Chaperone. Biochemistry 59, 1946-1960 (2020).
2019
Yang, H., Hofstetter, H., Cavagnero, S. Fast-Pulsing LED-Enhanced NMR: a Convenient and Inexpensive Approach to Increase NMR Sensitivity J. Chem. Phys. 151, 245102-245112 (2019).
Cavagnero, S. Exploring Biology and Embracing Diversity at SACNAS. BPS Bulletin, December issue (2019).
Varela A.E., England, K.A., Cavagnero, S. Kinetic Trapping in Protein Folding. Prot. Eng. Des. Sel. 32, 103-108 (2019).
Yang, H., Cavagnero, S. Improved sensitivity of laser-enhanced 1Hα–13Cα-correlation via suppression of Cα-C′ scalar-coupling evolution. J. Magn. Res. 307, 106572, 1-6 (2019).
Okuno, Y., Mecha, M.F., Yang, H., Zhu, L., Fry, C.G., Cavagnero, S. Laser- and Cryogenic-Probe-Assisted NMR Enables Hypersensitive Analysis of Biomolecules at Sub-Micromolar Concentration. Proc. Nat. Acad. Sci. USA 116, 11603-11611 (2019).
2018
Varela, A.E., Lang, J.F., Wu, Y., Dalphin, M.D., Stangl, A.J., Okuno, Y., Cavagnero, S. Kinetic Trapping of Folded Proteins Relative to Aggregates under Physiologically Relevant Conditions. J. Phys. Chem. B 122, 7682-7698 (2018).
Okuno, Y., Cavagnero, S. Effect of Heavy Atoms on Photochemically Induced Dynamic Nuclear Polarization in Liquids. J. Magn. Res. 286, 172-187 (2018).
2017
Okuno, Y., Cavagnero, S. Photochemically Induced Dynamic Nuclear Polarization: Basic Principles and Applications. eMagRes 6, 283-313 (2017).
Carlson, T., Lam, K.W., Lam, C. W., He, J., Maynard, J.H., Cavagnero, S. Naked-Eye Detection of Reversible Protein Folding and Unfolding in Aqueous Solution. J. Chem. Ed. 94, 350-355 (2017).
Cavagnero, S. A Prize for Going in Vivo. BPS Bulletin, January issue (2017).
2016
Okuno, Y., Cavagnero, S. Fluorescein: a Photo-CIDNP Sensitizer Enabling Hyper-Sensitive NMR Data Collection in Liquids at Low Micromolar Concentration. J. Phys. Chem. B 120 12416 (2016).
2015
Lee, J.H., Zhang D., Hughes C.P., Okuno Y., Sekhar, A., Cavagnero S. Heterogeneous Binding of the SH3 Client Protein to the DnaK Molecular Chaperone. Proc. Nat. Acad. Sci. USA 112, E4206-4215 (2015).
2014
Jennaro, T.S., Beaty, M.R., Kurt-Yilmaz, N., Luskin, B.L., Cavagnero, S. Burial of Nonpolar Surface Area and Thermodynamic Stabilization of Globins as a Function of Chain Elongation. Proteins: Struct. Funct. Bioinf. 82, 2318-2331 (2014).
Lee, J.H., Okuno, Y., Cavagnero, S. Sensitivity Enhancement in Solution NMR: Emerging Ideas and New Frontiers. J. Magn. Res. 241, 18-31 (2014).
Fedyukina, D.V., Jennaro, T.S., Cavagnero, S. Charge Segregation and Low Hydrophobicity Are Key Features of Ribosomal Proteins from Different Organisms. J. Biol. Chem. 289, 6740-6750 (2014).
Cavagnero, S. Fascinated by Live Cells. BPS Bulletin, November issue (2014).
Gierasch, L., Cavagnero, S., Gruebele, M. Biophysics in the Cell Comes Alive at the BIV Symposium. BPS Bulletin, April issue (2014).
2013
Zhu, L., Kurt Yilmaz, N., Choi, J., Lapidus, L.J., Cavagnero, S. Sub-Millisecond Chain Collapse of the E. coli Globin ApoHmpH. J. Phys. Chem. B 117, 7868-7877 (2013).
Lee, J.H., Cavagnero, S. A Novel Tri-Enzyme System in Combination with Laser-Driven NMR Enables Efficient Nuclear Polarization of Biomolecules in Solution. J. Phys. Chem. B 117, 6069-6081 (2013).
Knight, A.M., Culviner, P.H., Kurt Yilmaz, N, Zou, T., Ozkan, S.B., Cavagnero, S. Electrostatic Effect of the Ribosomal Surface on Nascent Polypeptide Dynamics. ACS Chem. Biol. 8, 1195-1204 (2013).
Wittung-Stafshede P., Gierasch L., Cavagnero S. BIV Subgroup Takes Exciting Journey through in Vivo Biophysics. BPS Bulletin, April issue (2013).
2012
Sekhar, A., Lam, H.N., Cavagnero, S. Protein Folding Rates and Thermodynamic Stability are Key Determinants for Interaction with the Hsp70 Chaperone System. Protein Sci. 21, 1489-1502 (2012).
Sekhar, A., Santiago, M., Lam, H.N., Lee, J.H., Cavagnero, S. Transient Interactions of a Slow-Folding Protein with the Hsp70 Chaperone Machinery. Protein Sci. 21, 1042-1055 (2012).
Cavagnero, S., Mukerjee, I. Getting Places Together: How to Find and Keep a Good Mentor. BPS Bulletin, April issue (2012).
2011
Kurt, N., Mounce, B., Ellison, P.A., Cavagnero, S. Residue-Specific Contact Order and Contact Breadth in Single-Domain Proteins: Implications for Folding as a Function of Chain Elongation. Biotech. Progr. 28, 297-297, correction (2011).
Lee, J.H., Sekhar, A., Cavagnero, S. 1H-Detected 13C Photo-CIDNP as a Sensitivity Enhancement Tool in Solution NMR. J. Am. Chem. Soc. 133, 8062-8065 (2011).
Armstrong, B.D., Choi, J., López, C., Wesener, D.A., Hubbell, W., Cavagnero, S., Han, S. Site-Specific Hydration Dynamics in the Nonpolar Core of a Molten Globule by Dynamic Nuclear Polarization of Water. J. Am. Chem. Soc. 133, 5987-5995 (2011), (*) corresponding authors.
Fedyukina, D.V., Cavagnero, S. Protein Folding at the Exit Tunnel. Annu. Rev. Biophys. 40, 337-359 (2011).
Rajagopalan, S., Kurt, N., Cavagnero, S. High-Resolution Conformation and Backbone Dynamics of a Soluble Aggregate of Apomyoglobin119. Biophys. J. 100, 747-755 (2011).
2010
Fedyukina, D.V., Rajagopalan, S., Sekhar, A., Fulmer, E.C., Eun, Y.-J., Cavagnero, S. Contribution of Long-Range Interactions to the Secondary Structure of an Unfolded Globin. Biophys. J. 99, L37-L39 (2010).
Weinreis, S.A., Ellis, J.P., Cavagnero, S. Dynamic Fluorescence Depolarization: A Powerful Tool to Explore Protein Folding on the Ribosome. Methods 52, 57-73 (2010).
Ellis, J.P., Culviner, P.H., Cavagnero, S. Confined Dynamics of a Ribosome-Bound Nascent Globin: Cone Angle Analysis of Fluorescence Depolarization Decays in the Presence of Two Local Motions. Protein Sci. 19, 1600 (2010).
Ziehr, D.R., Ellis, J.P., Culviner, P.H., Cavagnero, S. Production of Ribosome-Released Nascent Proteins with Optimal Physical Properties. Analyt. Chem. 82, 4637-4643 (2010).
2009
Ellis, J.P., Culviner, P.H., Cavagnero, S. Confined Dynamics of a Ribosome-Bound Nascent Globin: Cone Angle Analysis of Fluorescence Depolarization Decays in the Presence of Two Local Motions. Protein Sci. 18, 2003-2015 (2009).
Sekhar, A., Cavagnero, S. EPIC- and CHANCE-HSQC: Two 15N-photo-CIDNP-enhanced pulse sequences for the sensitive detection of solvent-exposed tryptophan. J. Magn. Res. 200, 207-213 (2009).
Sekhar, A., Cavagnero, S. 1H Photo-CIDNP Enhancements in Heteronuclear Correlation NMR Spectroscopy. J. Phys. Chem. 113, 10548-10548 (2009).
2008
Mounce, B., Kurt, N., Ellison, P.A., Cavagnero, S. Nonrandom Distribution of Intramolecular Contacts in Native Single-Domain Proteins. Proteins: Struct. Funct. Bioinf. 75, 404-412 (2008).
Ellis, J.P., Bakke, C.K., Kirchdoerfer, R.N., Jungbauer, L.M., Cavagnero, S. Chain Dynamics of Nascent Polypeptides Emerging from the Ribosome. ACS Chem. Biol. 3, 557-566 (2008).
Kurt, N., Mounce, B., Ellison, P.A., Cavagnero, S. Residue-Specific Contact Order and Contact Breadth in Single-Domain Proteins: Implications for Folding as a Function of Chain Elongation. Biotech. Progr. 24, 570-575 (2008).
Kurt, N., Cavagnero, S. Nonnative Helical Motif in a Chaperone-Bound Protein Fragment. Biophys. J. 94, 48-50 (2008).
Eun, Y.-J., Kurt, N., Sekhar, A., Cavagnero, S. Thermodynamic and Kinetic Characterization of ApoHmpH, a Fast-Folding Bacterial Globin. J. Mol. Biol. 376, 879-897 (2008).
2007
Kirchdoerfer, R.N., Huang, J.-T., Isola, M.K., Cavagnero, S. Fluorescence-Based Analysis of Aminoacyl- and Peptidyl-tRNA by Low-pH Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis. Analyt. Biochem. 364, 92-94 (2007).
2006
Vega, C.A., Kurt, N., Chen, Z., Rüdiger, S., Cavagnero, S. Binding Specificity of an α-Helical Protein Sequence to a Full-Length Hsp70 Chaperone and Its Minimal Substrate-Binding Domain. Biochemistry 45, 13835 -13846 (2006).
Chen, Z., Kurt, N., Rajagopalan, S., Cavagnero, S. Secondary structure mapping of DnaK-bound protein fragments: chain helicity and local helix unwinding at the binding site. Biochemistry 45, 12325-12333 (2006).
Cavagnero, S., Kurt, N. Folding and Misfolding as a Function of Polypeptide Chain Elongation: Conformational Trends and Implications for Intracellular Events. In Misbehaving Proteins: Protein (Mis)Folding, Aggregation, and Stability, ed. A.M. Tsai and R.M. Murphy, Springer, 217-246 (2006).
Jungbauer, L.M., Cavagnero, S. Characterization of Protein Expression and Folding in Cell-Free Systems by MALDI-TOF Mass Spectrometry. Analyt. Chem. 78, 2841-2852 (2006).
Jungbauer, L.M., Bakke, C.K., Cavagnero, S. Experimental and Computational Analysis of Translation Products in Apomyoglobin Expression. J. Mol. Biol. 357, 1121-1143 (2006).
Ellison, P.A., Cavagnero, S. Role of Unfolded State Heterogeneity and En-route Ruggedness in Protein Folding Kinetics. Protein Sci. 14, 564-582 (2006).
Bakke, C.K., Jungbauer, L.M., Cavagnero, S. In Vitro Expression and Characterization of Native Apomyoglobin under Low Molecular Crowding Conditions. Protein Expr. and Purif. 45, 381-392 (2006).
Kurt, N., Rajagopalan, S., Cavagnero, S. Effect of Hsp70 Chaperone on the Folding and Misfolding of Polypeptides Modeling an Elongating Protein Chain. J. Mol. Biol. 355, 809-820 (2006).
Chow, C.K., Kurt, N., Murphy, R.M., Cavagnero, S. Structural Characterization of Apomyoglobin Self-Associated Species in Aqueous Buffer and Urea Solution. Biophys. J. 90, 298-309 (2006).
2005
Kurt, N., Cavagnero, S. The Burial of Solvent-Accessible Area is a Predictor of Polypeptide Folding and Misfolding as a Function of Chain Elongation. J. Am. Chem. Soc. 127, 15690-15691 (2005).
Cavagnero, S., Jungbauer, L.M. Painting Protein Misfolding in the Cell with an Atomic-Scale Brush. Trends in Biotechnology. 23, 157-162 (2005).
2004
Rajagopalan S, Chow, C.K., Fry, C., Ragunathan, V., Cavagnero, S. NMR Spectroscopic Filtration of Polypeptides and Proteins in Complex Mixtures. J. Biomol. NMR 29, 505-516 (2004).
2003
Chow, C.C., Chow, C.K., Rhagunathan, V., Huppert, T., Kimball, E., Cavagnero, S. Chain Length Dependence of Apomyoglobin Folding: Structural Evolution from Misfolded Sheets to Native Helices. Biochemistry 42, 7090-7099 (2003).
Cavagnero, S. Using NMR to Determine Protein Structure in Solution. J. Chem. Ed. 80, 125-127 (2003).